Simultaneous determination of doxorubicin and active metabolite doxorubicinol in plasma by capillary electrophoresis and field-amplified sample stacking

A field-amplified sample stacking and capillary electrophoresis with UV detection was described for the determination of doxorubucin and doxorubicinol in human plasma. After sample pretreatment, field-amplified sample stacking method was applied for sensitive improvement. The sample was employed with an electrokinetic injection of 10 kV for 30 sec. The capillary electrophoresis separation of doxorubicin and doxorubicinol in human plasma was performed at 25C using a background electrolyte consisting of phosphate buffer (50 mM, pH 6.5) containing 16% ethylene glycol and methanol 60%. Using metformin as an internal standard, the linear range of the method for the determination of doxorubicin and doxorubicinol was over 20.0-100.0 ng/mL. The limit of detection of the doxorubibin and doxorubicinol in human plasma was 5 ng/mL(S/N=3, 10 kV, 30 sec) and 10 ng/mL (S/N=3, 10 kV, 30 sec) respectively. For optimization of the procedure, the pH and strength of phosphate buffer, the volume of ethylene glycol and methanol would be discussed. Application of the method to the determination of doxorubicin and doxorubicinol in human plasma proved to be feasible. © 2015 Universal Research Publications. All rights reserved